Journal: Analytical Chemistry
Article Title: Transient Cross-linking Mass Spectrometry: Taking Conformational Snapshots of Proteins
doi: 10.1021/acs.analchem.4c04939
Figure Lengend Snippet: (A) Cross-link identification and software evaluation. (B) SASD measurement of cross-links from the training group. (C) DCD cross-linking of the E. coli 70S ribosome; blue lines indicated cross-links having SASD ≤ 31.86 Å, and orange red lines indicated cross-links having SASD > 31.86 Å. (D) Number of valid cross-links (indicating those having the reporter ion and satisfying the ambiguous site annotation criterion ( n – n , n ≤ 3), 113 cross-links), number of cross-linked proteins (34 proteins), and type of cross-link products (79 intramolecular and 34 intermolecular cross-links).
Article Snippet: Escherichia coli 70S ribosome was purchased from New England Biolabs (Beverly, Massachusetts, USA).
Techniques: Software